BIOETHANOL PRODUCTION AND OPTIMIZATION TEST FROM VEGETABLE WASTE USING SACCHAROMYCES CEREVISIAE

Name of proposal (MSME FUNDED: 4.25 LAKHS, TOTAL COST: 5 LAKHS)
NAME OF INCUBATEE: MR. DIPTIKANTA ACHARYA

BASIS AND PRESUMPTIONS:-

The basis for calculation of production capacity of Bioethanol has been taken on single shift basis on 95% efficiency.
The maximum capacity utilization on single shift basis for 300 days a year. During first year and second year of operations the capacity utilization is 60% and 80% respectively. The unit is expected to achieve full capacity utilization from the third year onwards.
The salaries and wages, cost of raw materials, utilities, rents, etc. are based on the prevailing rates in and around Gunupur. These cost factors are likely to vary with time and location.
Interest on term loan and working capital loan has been taken at the rate of 13% on an average. This rate may vary depending upon the policy of the financial the policy of the financial institutions/agencies from time to time.
The cost of machinery and equipments refer to a particular make/model and prices are approximate.
The break-even point percentage indicated is of full capacity utilization.
The project preparation cost etc. whenever required could be considered under pre-operative expenses.
The production unit may also utilize common test facilities of Bioethanol which are available at CIPET, Bhubaneswar and other Biotech research laboratory of Odisha.

AIM AND OBJECTIVES:-

The main objective of this study is to determine the potential production and optimization of bioethanol from vegetable wastes.
To study the optimum hydrolysis of vegetable waste with diluted sulfuric acid and distilled water, and determining the influence of acid concentration and retention times

MATERIALS AND METHODS:-

Sample preparation: The vegetable wastes (from potato, brinjal, ladies finger, cabbage, onion, etc.) were collected from G.I.E.T Hostels and Canteen and then taken to Laboratory of Dept. of Biotechnology, G.I.E.T, Gunupur, for analysis.
Determination of Moisture Content:
Hydrolysis
Fermentation
Microorganisms
Analytical Methods
Determination of sugar content
Gas chromatographic determination of bioethanol

OUTCOMES OF THE PROJECT:-

Vegetable waste is one of the potential Biomass resources for bioethanol production. Bioethanol as a source of energy will be more than complementing solar, wind and other renewable energy sources in the long run.

UNIQUENESS OF THE WORK:-

Earlier works for bioethanol production was with different raw materials. This innovation work is emphasized on production of bioethanol by taking vegetable wastes. This is the uniqueness of the process.

UTILITY OF THE OUTCOME PRODUCTS(AT LARGE):

Bioethanol can bee produced in large scale by using large tank size with capacity of more than 1000 gallons

DISCUSSIONS:-

The result showed that the amount of sugar obtained decreases as the acid concentration increases and reaches maximum in acid free hydrolysis.
Acid concentration of 1, 2 and 4% H2SO4 for 1h hydrolysis, resulted the ethanol concentration of 6.097, 4.395 and 3.323 g/L, respectively. The maximum ethanol concentration of 6.315 g/L was obtained from vegetable waste hydrolyzed with distilled water, which is an optimum condition.
The concentration of ethanol obtained by the hydrolysis of the vegetable waste using distilled water, which is about 7.4 g/L was highly satisfactory.

CONCLUSIONS:-

Vegetable waste is promising lignocellulosic feed stocks for bioethanol production which can be used as a future biofuel.